Place it as the closing beat of a helpful moment, right after the good deed lands and before the next scene starts. Follow it with the Yaaaay Cheer Sound to turn a simple thanks into a small celebration, or use the Bruh Soundboard Button first for a sarcastic setup before the thanks arrives.
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This short clip is Mario saying 'Thank you so much!' in his bright, cheerful voice, squeezed through a bitcrushed effect that makes every word sound crunchy, retro and slightly glitchy. It lasts about 2.6 seconds. The warmth of the thanks stays clear underneath the distortion, so it feels playful, grateful and a little silly rather than sincere in a formal way.