Place it at the reveal point of a horror cut, after a Danger Alarm Meme Sound Button builds the warning, then follow it with the FNAF Jumpscare Scream for a second scare or a Roblox Oof Sound Button to flip into comedy.
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This clip is a 4.9-second jumpscare effect built to land as a sudden scare cue. It gives a listener a sharp jolt of tension and alarm, the kind of startled reaction horror games use when something appears without warning. It comes from Mary's Arcade, matching the arcade horror setting in its name.