Place it at the end of a betrayal scene, after a danger alarm, then cut straight to a FNAF jumpscare scream, or follow it with a No God Please No from the victim.
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This is a roughly two-second clip of Monokuma, the black-and-white robotic bear from Danganronpa, letting out his shrill, giggling laugh. It sounds cheerful at first, then turns unsettling, as if he is delighted by someone else's bad luck. The laugh comes from Danganronpa, the Spike Chunsoft visual novel series that began in Japan in 2010.