The exact origin of this clip is undocumented, and it circulates as a user-uploaded meme sound rather than a line from a known show or film. Its appeal is the single-joke clarity: the horse's 'no' needs no translation, so creators reuse it wherever a debate needs a hard full stop. Short meme sounds like this travel through soundboards, where small communities trade and rename clips, and this one has survived on that simple, repeatable gag. It endures because the joke lands in under two seconds.