Put it at the end of a failed-plan scene, just before the escape begins, then follow it with the Cartoon Run Take Off sound as the chase starts and close with the JoJo To Be Continued sound for the freeze-frame payoff.
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The clip is Joseph Joestar shouting 'Nigerundayo!' in a loud, urgent voice, stretched across just under four seconds as the word rushes out in one breath. It feels panicked and funny at once, like a dramatic escape call delivered with total commitment. The line is Joseph's famous warning from JoJo's Bizarre Adventure, shouted when retreat is the only sensible plan.