Put it just before the reveal, after a Dun Dun Dun cue, then follow it with a Vine Boom for the payoff. Keep it as the only jumpscare in the sequence so the pairing stays clear.
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This four-second clip is a single drawn-out scream, one long 'Aaaah' carried by a single voice. It feels sudden and startling, the kind of noise that makes a listener jump before they have time to laugh at themselves. It works as a pure shock sound, built around that one startled cry.