Place it right after the reveal that changes everything, following Sudden Suspense to build tension, then let Anime Punch land the impact once it finishes and the scene needs a sharp comic payoff.
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This is a nearly 39-second vocal reaction built around the Japanese exclamation 'oh kami yo', delivered as a drawn-out cry of disbelief. It feels dramatic, exasperated and overwhelmed, like someone watching a plan collapse in slow motion and appealing to the heavens for help.