Place it at the cut into a new room, before any reaction or scare, then follow it with the Roblox Oof when a run ends badly or the FNAF Jumpscare Scream when the reveal turns into a fright.
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This is a very short door opening effect, lasting under a second, with a quick creak and swing that signals moving into another room. It feels tense and expectant, as if something could be waiting just beyond the doorway. It comes from Roblox Doors, the horror game where players advance room by room, so the cue instantly reads as the next stage of a run.