Place this clip at the end of a short reaction sequence, after the setup has played out. Follow a failed attempt with the Womp Womp Fail first, then add this laugh as the closer, or lead into it with the Boing Boing for cartoon pranks.
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This clip is a very short burst of Polka delivering her drawn-out 'uheeheeey' giggle in a bright, high-pitched voice. It lasts just over a second and feels mischievous and silly, like a gremlin laugh after a prank has landed. The exaggerated, squeaky delivery makes it instantly recognisable as a playful reaction rather than a normal laugh.