Place it at the exact fail frame, then follow with the Emotional Damage Meme Sound if the moment needs a second hit, or swap in a Bruh Sound Effect for a drier follow-up from another player in the same clip.
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This 1.4-second clip is Kirby stretching his usual Poyo into a long, strained Poyooo, high-pitched but pushed into a gruff shout. It feels cute and fed up at once, turning mild annoyance into a playful outburst instead of real anger. It comes from Nintendo's round pink hero Kirby, known for his soft Poyo calls.