Place it near the end of a short fail or recovery moment, after the slip and just before the reaction shot. Follow it with Anime Wow for a surprised face, or lead into it with Mario Jump when a character springs back up, keeping the payoff clear.
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Poyoyoi is a short, springy vocal sound effect that stretches its playful syllables across about six seconds, with a light, high-pitched delivery that feels like a cartoon bounce. It gives a listener a quick lift, turning a small surprise or a cute moment into something silly and cheerful, without ever sounding harsh or heavy.