There is no widely documented original source for this particular human quack, and it appears to circulate as a short user-made soundboard clip rather than a line from a film, show or game. That kind of homemade animal impression has long been popular online, because it is easy to recognise, easy to reuse and instantly reads as a joke without needing context. In short-form videos and livestreams it is often used as a quick reaction noise, a punchline for absurd moments or a playful way to interrupt a serious scene.