Place it just before the reveal in a prank or horror edit, then follow it with the FNAF 2 jumpscare scream for the payoff, or use why are you running first to build the chase. A shocked reaction afterwards closes the scene.
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This short clip is a low male voice speaking right behind the listener, close and sudden enough to feel like someone has crept up unnoticed. It lasts about two seconds, with the voice alone carrying the threat and no music or other effect to soften it. The feeling is tense and startling, a quick jolt that makes the listener turn round before they have time to think.