Place it just before the reaction shot, then follow it with a FNAF Jumpscare Scream or a Wet Fart Sound Effect to keep the bathroom joke going in the next beat.
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This is a brief, loud scream of just over three seconds, performed as if someone has yelled without warning inside a tiled public restroom, with the echo carrying the shout. It feels startling and awkward at first, then ridiculous, because the noise is far too big for such a quiet, private place. That clash makes it land as a fast burst of public embarrassment.