Place it exactly where the reveal happens, then cut to the Oh My God Meme Sound as the next reaction and the Scream Meme Sound if another shock follows immediately.
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This is a brief, just-over-two-second outburst from SMG4, a high, frantic yell that bursts out all at once. It feels over-the-top and comic rather than truly frightening, turning a sudden shock into instant alarm. The clip comes from SMG4: If Mario Was in Splatoon 2, where Mario enters the world of Splatoon 2.