Place it at the end of a short fail sequence, after the mistake is clear and before the reaction shot. Follow it with the Metal Gear Alert Sound to mark the danger, or lead into it with the DJ Airhorn Sound Effect for a bigger payoff at the end of the sequence.
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This is a sharp, roughly one-second scream from Charlie, one of the main characters in Smiling Friends, delivered in a frantic, strained cartoon voice. It feels like sudden, exaggerated panic, funny rather than frightening, and lands as pure disbelief. The clip comes from Smiling Friends, the adult animated series created by Zach Hadel and Michael Cusack.