The clip comes from The Spectacular Spider-Man, the animated series that ran from 2008 to 2009 and starred Josh Keaton as Peter Parker. The show became a fan favourite for its sharp writing and comic-accurate take on Spider-Man, and short voice lines from it later spread as reaction sounds on meme pages and soundboards. This one survived because it works without context, a quick wrong answer buzzer delivered in character instead of a generic effect, which keeps it popular for fail edits and joke corrections.