Put it at the opening of a stream highlights edit, before the chat reaction shots, then follow it with a bell ding to close the shout-out. For a milestone sequence, save the mlg air horn for after it, so the celebration builds instead of peaking immediately.
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This is a 9-second Twitch alert sound effect built as a mega cue for new followers, donations and subscriptions. It gives a stream an upbeat, rewarding lift, turning a routine notification into a small celebration that thanks the supporter and energises the chat in the moment.