Place it at the start of a boss-intro sequence, then follow it with the Inception Horn for scale before cutting to gameplay, and save the Enemy Spotted clip for the moment the fight actually begins.
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A short 2.7-second voice clip of Trobbio, the flamboyant showman from Hollow Knight: Silksong, delivering one of his theatrical lines in a dramatic, performative tone. It feels playful and over-the-top, like a stage performer announcing himself before a duel, and it comes straight from the game, making it instantly recognisable to players of the game.