Place this clip right after the mistake appears on screen, before any commentary, then follow it with a huh reaction for a second beat. For a bigger escalation, layer in an oh my god wow moment after it instead of adding more effects underneath.
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This is a short 1.8-second vocal clip of someone letting out a drawn-out 'uh' that slides into a groaning 'ogh.' It sounds caught off guard and mildly pained, the kind of noise you make when you realise something has gone wrong. The feeling is awkward and funny rather than serious, perfect for an instant confused reaction.