Place it just before the unicorn reveal in an edit, then follow it with a yaaaay cheer once the unicorn is on screen, keeping the unicorn moment at the centre of the clip.
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This is a short clip of about 2.6 seconds built around the single word Unicoooooorn, stretched out with extra vowels in the title. The drawn-out spelling gives it a light, silly, playful feel, like a quick call made to get a smile. It works as a brief reaction rather than a longer line or song.