Put it just after the stranger appears, then follow it with Enemy Spotted to name the intruder before the confrontation, and save FBI Open Up for the moment someone tries to enter. Keep it early in a gaming montage so the challenge sets up the next scene.
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This is a short spoken line in which a voice asks, 'Who are you?', delivered in a direct, questioning tone over about four seconds. It feels suspicious and confrontational, like catching a stranger somewhere they should not be, with a dry, tense edge that makes the challenge land instantly. The line comes from Half-Life.