Place it immediately after the false claim, so the denial becomes the turning point of the scene. Follow it with Awkward Moment to let the exposure hang, or with Womp Womp Fail when the liar's excuse collapses completely. Keep it as the single accusation before the reaction shot.
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This is a short Japanese voice line from Danganronpa, lasting about 1.8 seconds, in which a speaker declares 'It's a lie!' with sudden, emphatic energy. It feels dramatic and accusatory, like catching someone mid-bluff, while still keeping the playful, over-the-top tone of the series.