Place it at the end of an interruption, after the other person has spoken, so it answers them directly. Pair No No Wait Wait before it to build the refusal, then follow it with Oh My God Wow for the reaction shot.
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A short Portuguese voice clip, about two seconds long, in which a voice says “Deixe-me em paz, homem” — leave me alone, man. It lands as a blunt, fed-up brush-off, and that tired irritation is what makes it funny, especially when it cuts off someone who keeps talking and will not take the hint.