Put it right after the wrong answer is revealed, as the closing beat of that joke, then follow it with the Nope Meme Sound to underline the rejection before the next question starts.
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This under-a-second clip is the harsh buzz that marks a wrong answer on Family Fortunes, with a flat electronic tone that cuts straight through. It feels instantly funny rather than cruel, turning a bad guess into a light punchline everyone recognises at once. It comes from Family Fortunes, the long-running British television game show.