Place this clip right after the visual punchline in a short edit, then follow it with the Mission Failed Sound Button for a bigger defeat or the Ba Dum Tss Sound Button when the fail is meant as a light joke.
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This clip is a short, roughly two-second human yell pitched high and stretched into a cartoonish, goofy wail. There are no words, just an exaggerated scream that climbs in tone. It feels silly and startled rather than scary, giving a moment of panic a light, funny and over-the-top energy that listeners instantly read as a joke.