Place it at the exact frame where a calm scene tips into panic, after a build-up beat and before the reaction shot. Follow it with No No Wait Wait to extend the scramble, or precede it with Sudden Suspense when the edit needs a setup first.
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This is a 2.5-second voice clip of Guido Nicheli delivering the Italian word 'agitato' as a single pointed exclamation. It gives a listener a flustered, theatrical feeling, like someone announcing they are rattled rather than calmly explaining it, which makes the brief outburst land as comic exasperation instead of genuine anger.