Place it at the very end of a roast exchange, after the other person's line has finished. For a longer bit, open with the shut up mom clip for the first rude reply, then close with this one as the escalated final answer.
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This 2.6-second voice clip captures the line 'Hey! Bite my glorious golden ass!' delivered as a bold, over-the-top retort. It feels defiant, cheeky and instantly funny, turning a simple insult into a theatrical put-down. The clip comes from Futurama Season 4 Episode 15, The Farnsworth Parabox.