Place it at the start of a Karl entrance, then follow with the Vine Boom for a heavier reaction and finish with the Dun Dun Dun sting when his plan goes wrong. That order keeps the joke focused on Karl before the edit moves on.
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This is a short, stretched-out voice calling out the name Karl in a rising, exasperated tone, lasting just over a second. It feels absurd and instantly familiar, like catching a friend mid-rant before the joke lands. The line comes from Llamas with Hats, where the llamas' deadpan exchanges turn a simple name into a running gag.