Place it just before the clever idea is revealed, then follow the payoff with Long Brain Fart when the idea turns out to be nonsense, or with Bruh when the audience is meant to doubt it. Keep it as the setup line, not the punchline, so the paired reaction carries the joke.
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This short Italian voice clip states the line about oxygen enlarging the brain in about four seconds. The absurd claim sounds like a confident piece of folk wisdom, which gives it a dry, teasing humour. It works as a knowing nod to a clever remark, a strange idea, or a moment that suddenly feels smarter than it should.