Place this clip right after the on-screen accusation in your edit, then follow it with the Damn meme sound for the reaction beat. If the moment needs a build-up, put a No No Wait Wait button before it so the denial arrives as the payoff.
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A 3.3-second voice clip of Markiplier flatly insisting he is not a masochist, delivered as a firm, deadpan denial. It lands as defensive and funny, the kind of over-serious correction that makes the accusation sound even more ridiculous, which is why the line works as an instant comeback.