Place it near the end of a pet fail edit, after the setup and before the reveal, then follow it with a sad meow song for a second beat before a cat laugh meme breaks the sequence, or open with emotional damage for a bigger fall.
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This is a roughly nine-second clip of a cat meowing in a low, drawn-out and mournful way, with the repeated sound giving it a pleading, almost human quality. It feels sad and a little pitiful, the kind of sound that makes a small disappointment feel dramatic, while still staying cute enough to make listeners smile at the cat's exaggerated sorrow.