Place this clip at the reveal point of a short edit, after you have set up the sibling joke, then follow it with the Onii-chan Onii-chan button for a call-back and the Yamete Kudasai button as the reaction that closes the sequence for viewers.
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A four-second anime voice clip built around a sweet-sounding girl calling out to her onii-chan and then blurting out the crude punchline from the title. The contrast between the cute delivery and the blunt wording makes it feel awkward, cheeky and deliberately shocking, like a joke meant to catch listeners completely off guard in conversation.