Place it at the end of a failed attempt or wrong answer, after the setup has played out. Follow it with a Vine Boom for the next beat, or a SpongeBob Fail for a sillier reaction, letting the buzzer mark the mistake before the next joke begins.
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This is a short 2.4-second buzzer sound effect, a harsh electronic buzz that cuts in suddenly and stops just as fast. It feels abrupt and comically final, like a game-show signal that an answer was wrong, turning a confident moment into an instant fail.