Scooby Doo's laugh comes from the long-running Scooby-Doo animated series created by Hanna-Barbera, where the Great Dane's speech and reactions became central to the show's comedy. Voiced originally in a distinctive, slurred style, his laugh reflects his nervous but good-natured personality during mystery scenes. Clips of the laugh later spread online as a standalone reaction sound on soundboards and in meme edits, detached from full episodes. It remains popular because it is instantly recognizable to multiple generations and works without any dialogue or context.