Place it at the end of a failed chase or a stolen objective, after Enemy Spotted sets up the threat and before Nope closes the moment with a flat refusal. That order keeps the joke on the escape itself, and works best when the laugh is the last sound before the next play begins.
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This clip is Slark's brief laugh from Dota 2, a sharp burst of voice from the stealthy hero that runs for just under three seconds. The sound rises quickly, sounding amused and sly, then cuts off before it can grow louder. It gives a listener a cheeky, taunting feeling, as if someone has just slipped away unpunished, and it comes from Slark's voice lines in the Valve game.