Put it near the end of a scare sequence, after the Metal Gear Alert, so the alert sets up the moment and the scream closes it. Follow it with the DJ Airhorn as a second beat, rather than stacking another scream straight after.
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This is a short, high-pitched scream lasting about a second and a half, sharp and sudden with no build-up. It lands as a jolt, startling at first and then funny once the shock passes, which makes it useful for jump scares and exaggerated reactions.