Place it at the end of an exchange as the final beat, then follow it with the Classic Fart Sound for a second payoff before cutting away. For a bigger reaction, put the Vine Boom Sound just before it so the contrast makes the closing moment hit harder.
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This is a brief sound-effect clip titled Testes, running for just under a second. It delivers its single idea in one quick burst, with no build-up or extra detail to sit through. The effect is blunt and awkward, the kind of sudden gag that catches listeners off guard and gets a laugh precisely because it is so direct and unexpected.