Place it right after the offending remark in a reaction edit, then follow with Oh Brother This Guy Stinks for a bigger crowd reaction, or cut to Ba Dum Tss when you want the moment to end on a joke instead.
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This is a very short voice clip of Donald Trump saying 'That's not nice' in a flat, disapproving tone, lasting just over a second. It lands as a dry, mock-scolding rebuke, funny because the mild wording sounds deliberately restrained coming from him, and it works best as a quick, pointed response to rude or over-the-top behaviour.