Place this clip right after a reveal or mistake in your edit, then follow it with the shocked meme sound for a bigger reaction or the oh no no no laugh when the moment turns into a joke instead.
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This clip is a short human voice blurting out 'uou' in just under two seconds, a single drawn-out exclamation with nothing else behind it. Hearing it feels playful and caught-off-guard, like a cartoonish double-take, which makes the surprise land as funny rather than serious or startled in a tense way.