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Place it at the reveal in a horror edit, after a Baby Laughing Meme has opened the clip, then follow it with a scream for the jumpscare peak. Keep it as the turning point, not the opener, so the next sound hits harder.
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This is a brief, bent-out-of-shape laugh lasting just over two seconds, pitched sharp and frantic, like a cartoon giggle played through a broken file. It starts amusing, then feels wrong and threatening, as if a cheerful character has snapped. It comes from SpongeBob Plug 'n' Plays With Shrek by AquaticNeptune, clipped for horror-meme use.
