Put it at the end of a failed attempt, after the mistake is shown, so it closes the gag. For a bigger build-up, start with a dramatic dun dun dun, then let this clip finish the moment. If the fail is especially silly, follow it with the SpongeBob fail for a second punch.
关于此声音
This clip is a brief, about-five-second run of cartoon brass horns that sound after something has gone obviously wrong. It feels playful and silly rather than harsh, turning a miss into a joke instead of a punishment, and its familiar tone tells listeners at once that the failure is lighthearted and meant to be laughed at.