Place it right after the offending line or prank lands, then follow it with Oh My God Wow for the double-take, or cut to an Anime Punch when the joke turns into a playful fight.
关于此声音
This two-second clip captures Fluttershy, the soft-spoken pony, delivering a startled 'how dare you' in her gentle voice, suddenly sharp with disbelief. It feels sweetly indignant, more hurt than angry, like a polite friend finally pushed too far. The line comes from My Little Pony, where Fluttershy's quiet kindness makes her rare outburst land harder.