This clip is listed without an artist, film or character attached to it, so it is best understood as a generic reaction scream rather than a line from a known movie. Its short length and clear rise from eeeee to aaaaa make it easy to recognise and reuse, and that simplicity has helped it circulate as a sound for memes, short videos and chat reactions. Creators tend to reach for it when they need instant panic without dialogue, and its exaggerated tone keeps it comic rather than alarming, which explains why it gets used for fails and surprises.