The song is best known to many viewers through Sister Act, the 1992 comedy in which Whoopi Goldberg's character helps a convent choir find its voice, turning traditional hymns into joyful gospel performances. I Will Follow Him became one of the film's most memorable choir numbers, with its bright harmonies and clapping rhythm capturing the nuns' newfound confidence. Clips of the performance have since circulated online as a shorthand for celebration and devotion, and creators still reuse the short audio when they want an instantly recognisable, uplifting moment.