Place it right after a character is eliminated, then follow it with Law and Order Dun Dun to underline the loss. For a game over, put Mortal Kombat Fatality before it to set up the elimination, then add SpongeBob Sad Song after it for a mock mourning moment.
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This four-second South Park clip captures Stan shouting that Kenny has been killed, followed straight away by Kyle yelling 'You bastards!' in furious disbelief. The two young voices make it feel shocked, funny and deliberately over the top, like a cartoon disaster played for laughs. It comes from the show's running gag where Kenny dies in almost every early episode.