The Joker is Batman's most famous enemy in DC comics, films and animated series, and his exaggerated laugh has been part of the character for decades across many different actors and voices. Clips of that cackle spread widely on meme soundboards and short video edits, where it is used like other cartoon laugh effects such as a chipmunk laugh to signal mischief. Because audiences recognise the laugh instantly even without seeing the character, creators reuse it as a standalone punchline for villains, pranks and unhinged reactions.