Place it at the end of a fail sequence in your edit, then follow with Emotional Damage for the aftermath and Bruh when the disbelief needs a flat, deadpan close.
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Vegeta holds one desperate 'no' for just under three seconds, letting the shout break, wobble and stretch far past a normal refusal. The over-the-top panic makes it funny instead of serious, like watching someone refuse to accept bad news out loud. It comes from Dragon Ball Z, where the scream has been clipped and shared for years as a reaction to disbelief.