Place it at the start of a sibling scene, then follow it with Onii-chan Onii-chan for a back-and-forth call, and finish with Tuturu as a second reply from the other character in the scene.
关于此声音
The Nii-Nii Ayane clip is a short voice recording lasting under a second, built around a single affectionate spoken Japanese call for an older brother. It feels sweet and teasing, like calling out to get an older brother's attention in a lighthearted moment.