The exact origin of this short clip is not clear from its title alone, so it is best known through its use as a reaction sound online. Short animal reactions spread quickly on soundboards and in short form edits because they can be reused for almost any surprised moment, much like an oh my god meme clip. The pufferfish visual gives this one its identity, turning a simple oh into a recognisable cue for puffing up, freezing or reacting to something unexpected in comedy videos and chats.